Sher-e-Bangla Agricultural University

Thesis paper


VARIABILITY, PATHOGENICITY AND IN VITRO MANAGEMENT OF BOTRYTIS CINEREA CAUSING BOTRYTIS GRAY MOLD IN CHICKPEA (CICER ARIETINUM L.)
 
N° Catalog: SAU200701_157
University: SAU
Department: Plant Pathology
Year: 2007
File: 1st part: sau200701_157.pdf
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ABSTRACT

A total of ten isolates of Botrytis cinereainfecting chickpea in most chickpea growing
areas of Bangladesh were characterized in terms of cultural, morphological,
physiological and pathogenicity. The isolates varied significantly in cultural,
morphological and pathogenic traits- colony color, shape, margin and texture;
production and arrangement of sclerotia on PDA medium. The optimum temperature
and pH for the mycelial radial growth of Botrytis cinereawere recorded at 20°C and
pH 4.5, respectively. The mycelial radial growth of all ten isolates increases with the
time for a certain period. No growth was observed at 35°C temperature. The pathogen
Botrytis cinereagrew well on CDA medium. The highest (79.17 mm) mycelial radial
growth was obtained on CDA. The quickest (5 days) sclerotia initiation was observed
on CDA and LDA culture media but the highest number (2.5xl04 ml-1) of spores
were counted on LDA medium. The length of conidia varied from 5.00 to 15.00 um.
Mean length of conidia was maximum 12.00 μm in isolate AHI-9 and minimum 7.50
mμ in isolate AHI-1. The breadth of conidia ranged from 5.00 to 10.00 μ The
highest mean breadth 8.25μm was observed in isolate AHI-9 and the lowest 6.00 μm
in isolate AHI-4. The isolates exhibited different reaction of highly susceptible to
resistant to a set of chickpea cultivars and AHI-9 and AHI-10 were found the most
virulent isolates among the others. The antagonist Trichoderma harzianumappeared
to be a good bio-control agent against Botrytis cinerea.Among the seven tested
fungicides namely- Bavistin 50WP (Carbendazim), CP-Zim 50WP (Carbendazim),
Sunphanate 70WP (Thiophanate methyl) and Rovral 50WP (Iprodione) were the most
effective fungicides to inhibit the mycelial radial growth of Botrytis cinereaat a lower
concentration (500 ppm).